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Introduction: The PSC program is designed for evaluating primer specificity in PCR by checking the matching ability between the primer and DNA template. This program can predict most of the possible amplicons including both the specific and non-specific PCR products...... |
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Introduction: Small RNAs of less than 40 nucleotides (nt) in length constitute a large family of tiny regulatory molecules, such as microRNAs (miRNAs), small interfering RNAs (siRNAs) and scanRNAs (scnRNAs). They function in diverse pathways in eukaryotes and play critical roles in eukaryotes. miRNAs and siRNAs induce translation repression and/or mRNA cleavage by binding to mRNAs...... |
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Introduction: Many biologists have a long-standing interest in biological robustness, going back to Fisher's work on dominance (1-3), and to Waddington's developmental canalization research (4,5), which is defined as the ability to maintain stable functioning in the face of various perturbations. Robustness is a fundamental and ubiquitous observed phenomenon in biological systems...... |
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Introduction: The dbSTSeq,abbreviated for database of Standard Transcript Sequence is focused on building a collection of revised transcript sequences based on officially released genomic DNA sequence of human and other model species. The transcript sequences are collected from the cDNA, mRNA and EST sequences in public domain. The polymorphisms, sequencing errors or contamination of vectors in the transcript sequences will be "masked" after mapped to a unique set of genomic DNA sequence...... |
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Introduction:This program is designed for evaluating the specificity of PCR primers based on known multiple factors including sequence similarity searching, stability at the 3'end of the primer, melting temperature, GC content, and number of binding sites between the primers and DNA templates. It can help the user to select optimal primers before running either standard or multiplex PCR reactions... |
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Introduction:Pick primer pairs sets from multiple DNA sequences ( Based on primer3 ) |
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